11. GAUCHER CELL :
• Distended (phagocytic cells) Gaucher cells are found in
Spleen, Liver, Bone marrow, Lymph nodes, Tonsils, Thymus,
Payers patches, alveolar septa & air spaces in Lung.
• It has a fibrillary type of cytoplasm (crumpled tissue paper).
• Gaucher cell is a large cell 100 micron in diameter bearing
one or more eccentrically placed nuclei. PAS staining is
positive
• EM finding: Fib cyto is elongated, distended lysosomes
containing stored lipid in stacks of bilayers.
19. SPLEEN
• Earliest sign of GD is Splenomegaly leading to Hypersplenism
(functional hyperactivity)
• Extent of enlargement depends on the mutation genotype.
(1226G / 84GG)
• Most common presenting – Bleeding manifestations ( Epistaxis,
Bruising & Prolonged Bleeding time)
• Mechanical obstruction due to massive Splenomegaly (Abd
discomfort, Pain ,Splenic Infarcts, nodules, subcapsular infarcts,
wedge shaped areas)
• Growth retardation in children – few cases
22. LIVER:
•Hepatomegaly : Portal & Sinusoidal infiltration of
Gaucher cells along with Fibrosis, Inflammation
and/or Ischemia.
•Oesophageal varices in few cases.
•Liver failure/Portal hypertention uncommon.
•Hepatocytes not affected
•LFT normal / slightly abnormal(SGOT<SGPT may be
elevated).
23. BONE: Types of Orthopaedic
complications
•Nonspecific Bone pain episodic
•Pseudoosteomyelitis accompanied by with fever,
leukocytosis & Increased ESR.
•Pyogenic Osteomyelitis
•Chronic pain a/w stiffness of large joints.
•Aseptic necrosis of head of Femur
•Pathologic fractures.
•Spinal malalignment
•Growth & Stature reduction due to spinal deformity
24. BONE cont:
•WIDE metaphyseal-diaphyseal region (ERLENMEYER
FLASK) deformity – bilateral
•Epiphyses are unaffected.
•Bone density reduced diffuse or localized
•RARER:
• Compression /collapse of vertebral bodies
• Spinal cord /Nerve involvement.
• Ischemia /cortical necrosis due to intraosseous
pressure caused by the lipid laden cells.
•MECHANISM IS STILL UNEXPLAINED!!!
27. LUNGS:
•Findings are grouped in 3 categories:
•Interstitial infiltration of Gaucher cells.
•Capillary plugging leading to Pulmonary
Hypertention
•Alveolar consolidation reminiscent of Pneumonia
(type2GD)
•X-ray shows Miliary pattern/or reticular nodular
infiltrate
28. KIDNEY:
•Gaucher cells have been found in glomeruli, cortex,
medulla & interstitium without significant renal
complications.
•Kidney involvement in GD is uncommon .
29. HEART:
•Constrictive Pericarditis &Rheumatic aortic Valve
Disease
•Infiltration of myocardial interstitium by Gaucher
cells.
•Calcification of mitral/aortic valves & ascending
aorta
31. CNS:
•Type 2GD - Cranial nerve/extrapyramidal tract
involvement are common
•Classic triad (Trismus, Strabismus & Retroflexion)
•Type3- in the 1st
year of life children present with
(Spasticity, Hyperreflexia, positive Babinski sign &
Seizures
32. HAEMATOLOGICAL
MANIFESTATIONS:
•Anemia & Thrombocytopenia due to Hypersplenism &
infiltration of Gaucher cells in Bone marrow.
•Pancytopenia
•Iron def Anemia due to bleeding ASSO of GD with
Autoimmune disorders &Hematologic malignancies
•PT / APPT prolonged
33. IMMUNE SYSTEM
•Impaired chemotaxis of neutrophils
•T lymphocyte deficiencies in spleen and blood
including NKT cells.
•Hypergammaglobulinemia
•Monoclonal gammopathies
•Diffuse immunoglobulin elevation
•Change of IgM to IgG and IgA as disease progresses.
•Multiple myeloma
•Other concomitant malignancies
•Hodgkin’s, Acute leukemias, CLL, CML
34. OBGY
•Delay of puberty
•Heavy menstrual bleeding
•Spontaneous abortions
•Stillbirths, neonatal deaths
•Aggravation of thrombocytopenia and anemia
during pregnancy.
35. OTHER
•Hypocholesterolemia due to increased catabolism of
LDL and HDL.
•Serum level elevations of –
• Angiotensin converting enzyme (ACE)
• Chititriosidase
• Macophage colony stimulating factor
• Soluble CD 14
• Ferritin
• Transcobalamin II
• Beta hexosaminidase
• Lysozyme
36. MOLECULAR BASIS -
•Glucocerebrosidase gene is located on Chromosome 1 at
band q21.
•Just downstream the active gene is a highly homologous
pseudogene.
•More than 50 mutations in the active gene.
•Most common is Point mutation.
•Others are inserions, deletions, splicing, gene
conversion, crossing over between the functional gene
and pseudogene.
37.
38. MUTATION 1226: A G
•Point mutation in exon 9
•Mild type of mutation
•75% Ashkenazi Jews and 36% non-Jews
•Late age of onset
•Protective against CNS involvement.
39. MUTATION 1448: T C
•Point mutation in exon 10
•Severe type of mutation
•Seen in all 3 types and Norbottnian variant
•Most common in type 2 and 3
•Gene conversion, unequal crossing over
40. MUTATION 84GG
•Extra guanine inserted into the cDNA at position 84
•Lethal type of mutation
•Second most common in Ashkenazi Jews
41. MUTATION IVS2 (+1)
•Splice site mutation where adenine is substituted by
guanine between exon 2 and intron 2.
•Lethal mutation
42. MUTATION 1604: G A
•Point mutation in exon 11
•Mild mutation, non neuronopathic
43. THERAPY
ENZYME REPLACEMENT THERAPY (ERT) WITH
RECOMBINANT ENZYMES – cerezyme, vpriv
BONE MARROW TRANSPLANTATION
GENE TRANSFER THERAPY
SUBSTRATE REPLACEMENT THERAPY (SRT) –
GLUCOSYLCERAMIDE SYNTHASE INHIBITORS.
MIGLUSTAT
ELIGLUSTAT