2. ANATOMY OF BREAST
ī Modified apocrine sweat glands.
ī Breast parenchyma ī 12 to 20
lobes.
ī Within each lobe â Lactiferous duct
- branches repeatedly ī leads to
no. of terminal ducts ī each leads
to a lobuleī contains multiple
acini/alveoli ī TDLU
(TERMINAL DUCT + LOBULE)
ī Spaces around the lobules and
ducts and between the lobes are
filled with fatty tissue, ligaments
and connective tissue ī
STROMA
4. NORMAL HISTOLOGY OF THE BREAST
ī 2 cell types â line ducts &
lobules.
1. Contractile MYOEPITHELIAL
CELLS ī lie on the BM ī
assist in milk ejection during
lactation & provides structural
support to the lobules
2. EPITHELIAL CELLS ī
Luminal â produce milk.
ī Epithelial & Myoepithelial cells
lie on the basement
membrane.
5.
6. NORMAL HISTOLOGY OF THE BREAST
ī2 types of breast
STROMA:
1. INTERLOBULAR
STROMA ī Dense
fibrous connective tissue +
adipose tissue.
2. INTRALOBULAR
STROMA ī Envelopes
the acini + hormonally
responsive fibroblast â like
cells + scattered
lymphocytes.
7.
8.
9.
10. ACUTE MASTITIS
ī First month of breast feeding.
ī Cracks / fissures in the nipple ī
portal of entry of bacteria.
ī Breast ī
erythematous,painful,fever +nt.
ī MORPHOLOGY: Staph. Inf.ī
localized area of inflammation.
Strep. Inf. ī Diffuse, spreading.
ī HPE: Involved breast tissue â
necrotic, neutrophil infiltration.
ī Treated with antibiotics,
continuous milk expression.
Rarely surgical drainage.
11.
12. PERIDUCTAL MASTITIS
ī Recurrent subareolar abscess/
Squamous metaplasia of lactiferous
ducts/ Zuska ds.
ī Painful erythematous subareolar mass.
ī 90% cases â assoc. with smoking ī
Vit.A def./toxic substances in smoke â
alters epithelial differentiation.
ī Recurrent cases â fistula occurs.
ī HPE : Keratinizing squamous
metaplasia of ducts. Keratin shed
from the cellsī plugs the ductal
system ī dilation & rupture of duct.
ī Periductal tissue ī keratin spill ī
chronic granulomatous inflammatory
response.
ī Treatment: En bloc surgical removal
of the involved duct, fistula.
Antibiotics for secondary bacterial
infection.
13. DUCT ECTASIA
ī 5th â 6th decade, multiparous women.
ī Cl.features: Poorly palpable periareolar
mass, thick white secretions from
nipple, skin retraction.
ī HPE: Dilated ducts filled by granular
debris ī numerous lipid-laden
macrophages, inspissation of breast
secretions, marked periductal and
interductal ( dense )infiltrate of
lymphocytes and macrophages, and
variable numbers of plasma cells.
ī Eventual fibrosis ī skin & nipple
retraction. Principal significanceī
produces an irregular palpable mass -
mimics the mammographic appearance
of carcinoma.
14. DUCT ECTASIA
īDilated duct with
surrounding fibrosis and
chronic inflammation.
Lumen of the duct ī
eosinophilic secretion &
markedly attenuated
epithelium.
15. FAT NECROSIS
ī Cl.features: H/o breast trauma / prior
surgery.
ī Painless palpable mass, skin thickening
or retraction, a mammographic
density, or calcifications.
ī Acute lesions ī hemorrhagic +
central areas of liquefactive fat
necrosis.
Subacute lesions - areas of fat
necrosis ī ill-defined, firm, gray-
white nodules containing small chalky-
white foci or dark hemorrhagic debris.
Central region of necrotic fat cells
ī intense neutrophilic infiltrate +
macrophages.
ī Proliferating fibroblasts + new vessels
+ chronic inflammatory cells surround
the injured area ī Giant cells,
calcifications, and hemosiderin appear
ī focus - replaced by scar tissue.
17. GRANULOMATOUS MASTITIS
ī Rare.
ī CAUSES:
1. Systemic granulomatous ds.ī
Sarcoidosis, Wegenerâs.
2. Granulomatous inf. d/t
Mycobacteria, Fungi.
ī GRANULOMATOUS LOBULAR
MASTITIS â Parous women,
confined to lobules, d/t
hypersensitivity reactions to the
antigens â expressed by the
lobular epithelium during
lactation.
18.
19. Benign alterations â in ducts &
lobules:
īDetected by mammography/incidental findings in
surgical specimens.
īBased on the risk of developing Breast Cancer â 3
groups:
20. FIBROCYSTIC CHANGE
īMost common benign ī Morphology:
breast condition. â3 principle changesâ
īPrimarily affects terminal
ductâlobular unit (TDLU).
ī Pathogenesis ī Obscure
â hormones (estrogen)
-play a role.
īClinical features
īļIncidence: 10 â 20 % of
adult women.
īļAge : 25 â 45 yrs.
īļUsually bilateral.
īļVague âlumpyâ
21. FIBROCYSTIC CHANGE â CYSTS
ī Dilation & unfolding of
lobules ī small cysts â coalesce
ī large cysts.
ī Unopened cysts ī turbid ,semi
translucent fluid ī brown/blue
colour ī BLUE â DOME CYSTS.
ī Lined by flattened atrophic
epithelium/metaplastic apocrine
cells (Abundant granular
eosinophilic cytoplasm + round
nuclei).
ī Calcification â common.
ī âMILK OF CALCIUMâ â
Mammographers
ī Diagnosis â confirmed â
disappearance of the cyst after
FNAC.
22. FIBROCYSTIC CHANGE - FIBROSIS
Cysts rupture
Secretory material
Adjacent stroma
Chronic inflammation,
Fibrosis
Palpable firmness of the
breast
23. FIBROCYSTIC CHANGE - ADENOSIS
īIncrease in the number of
acini per lobule.
īPregnancy ī Normal
physiologic adenosis.
īNonpregnant women ī
adenosis - focal change.
īAcini â enlarged,not
distorted (blunt-duct
adenosis).
īCalcifications â occasionally -
within the lumens.
ī Acini - lined by columnar
cells ī benign / atypical
features (âflat epithelial
atypiaâ) ī Earliest
recognizable precursor of
epithelial neoplasia
24. LACTATIONAL ADENOMAS
ī Palpable masses â
pregnant/lactating women.
ī Normal appearing breast tissue +
physiological adenosis +
lactational changes.
ī Exagerrated focal response to
hormones.
ī Gross appearance: Well
circumscribed mass - distinct
lobular configuration, yellowish
color, and marked vascularization.
C/s: Gray / tan. Necrotic changes
frequent.
ī HPE:Proliferated glands lined by
actively secreting cuboidal cells
25.
26. PROLIFERATIVE BREAST DISEASE
WITHOUT ATYPIA
īMammographic densities, calcifications, or as
incidental findings in specimens from biopsies.
īFound alone/assoc. with non prolif. breast
changes.
īLesions ī proliferation of ductal epithelium and/or
stroma without cytologic or architectural features
suggestive of carcinoma in situ.
28. Sclerosing Adenosis
ī Palpable mass, a radiologic
density, or calcifications.
ī No. of acini per terminal duct -
increased to double the number NORMAL
found in uninvolved lobules.
ī Normal lobular arrangement -
maintained.
ī Acini - compressed and distorted
in the central portions of the
lesion & characteristically dilated
ADENOSIS
at the periphery.
ī Myoepithelial cells - prominent.
29. Complex sclerosing lesion
ī Radial sclerosing lesion (âradial
scarâ) - commonly occurring
benign lesion ī forms - irregular
masses (mimic invasive
carcinoma)mammographically,
grossly, and histologically.
ī Central nidus of entrapped glands
in a hyalinized stroma with long
radiating projections into stroma.
ī Radial scar â misnomer (lesions -
not assoc. with prior trauma or
surgery)
30. Papillomas
ī Multiple branching fibro vascular cores, each with
a connective tissue axis lined by luminal and
myoepithelial cells.
ī Growth - within a dilated duct.
ī Epithelial hyperplasia and apocrine metaplasia -
frequently present.
ī Large duct papillomas - solitary, situated in the
lactiferous sinuses of the nipple.
ī Small duct papillomas - multiple - located deeper
within the ductal system.
ī > 80% of large duct papillomas ī nipple discharge.
ī Large papillomas ī torsion of stalk ī infarction
ī bloody discharge.
ī Intermittent blockage and release of normal breast
secretions or irritation of the duct by the papilloma
ī Non bloody discharge.
ī Others ī + nt as small palpable masses, or as
densities or calcifications seen on mammograms
31. Atypical ductal/lobular hyperplasia ī Cellular proliferation -
resembles carcinoma in situ - but lacks sufficient qualitative or
quantitative features for diagnosis as carcinoma.
32. ATYPICAL DUCTAL
HYPERPLASIA
ī Found in Bx specimens â done for
calcifications,mammographic
densities,palpable masses.
ī Relatively monomorphic
proliferation of regularly spaced
cells, sometimes with cribriform
spaces.Limited in extent, only
partially filling ducts.
Duct is filled with a mixed population of
cells ī oriented columnar cells at the
periphery and more rounded cells within
the central portion. Some of the spaces -
round and regular, the peripheral spaces -
irregular and slitlike ī Highly Atypical.
33. ATYPICAL LOBULAR HYPERPLASIA
ī Proliferation of cells ī the cells
do not fill or distend more than
50% of the acini within a lobule.
ī Atypical lobular hyperplasia ī
also involves contiguous ducts
through pagetoid
spread( discrete intraepidermal
proliferation of cells occurring
singly/ nests at all levels of the
epidermis) in which atypical
A population of monomorphic small,
lobular cells lie between the ductal
round, loosely cohesive cells partially fill
basement membrane and a lobule. Some intracellular lumens can
overlying normal ductal epithelial be seen
cells.