Human Reproduction Update, Vol.17, No.5 pp. 693– 705, 2011
Advanced Access publication on July 4, 2011 doi:10.1093/humupd/dmr021
The history of female genital tract
malformation classifications and
proposal of an updated system†
Pedro Acién * and Maribel I. Acién
Service of Obstetrics and Gynaecology, University Hospital of San Juan; Department of Gynaecology, ‘Miguel Hernández’ University, Campus
of San Juan, Alicante, Spain
*Correspondence address: Departamento/División de Ginecologı́a, Facultad de Medicina de la Universidad ‘Miguel Hernández’, Campus de
San Juan, 03550 Alicante, Spain. Tel: +34-965919385; Fax: +34-965919550; E-mail: acien@umh.es
table of contents
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Introduction
Objectives
Embryological considerations
Methods
Literature search strategy
Selection criteria
Data extraction
Critical appraisal
Results
Evolutionary analysis of the existing classifications.
Unifying terms and concepts
Critical analysis of the current classifications
Comments and proposition for an updated classification
Proposed updated embryological-clinical classification for female genitourinary malformations
Conclusions
background: A correct classification of malformations of the female genital tract is essential to prevent unnecessary and inadequate
surgical operations and to compare reproductive results. An ideal classification system should be based on aetiopathogenesis and should
suggest the appropriate therapeutic strategy.
methods: We conducted a systematic review of relevant articles found in PubMed, Scopus, Scirus and ISI webknowledge, and analysis of
historical collections of ‘female genital malformations’ and ‘classifications’. Of 124 full-text articles assessed for eligibility, 64 were included
because they contained original general, partial or modified classifications.
results: All the existing classifications were analysed and grouped. The unification of terms and concepts was also analysed. Traditionally, malformations of the female genital tract have been catalogued and classified as Müllerian malformations due to agenesis, lack of fusion, the absence of
resorption and lack of posterior development of the Müllerian ducts. The American Fertility Society classification of the late 1980s included seven
basic groups of malformations also considering the Müllerian development and the relationship of the malformations to fertility. Other classifications
are based on different aspects: functional, defects in vertical fusion, embryological or anatomical (Vagina, Cervix, Uterus, Adnex and Associated
Malformation: VCUAM classification). However, an embryological-clinical classification system seems to be the most appropriate.
†
An initial version of this paper was presented in the ESHRE 26th Annual Meeting, Rome, Italy, 27 –30 June 2010, during The Invited session 21: Congenital malformations of the female
genital tract.
& The Author 2011. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
For Permissions, please email: journals.permissions@oup.com
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Submitted on December 18, 2010; resubmitted on March 26, 2011; accepted on April 12, 2011
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Acién and Acién
conclusions: Accepting the need for a new classification system of genitourinary malformations that considers the experience gained from
the application of the current classification systems, the aetiopathogenesis and that also suggests the appropriate treatment, we proposed an update
of our embryological–clinical classification as a new system with six groups of female genitourinary anomalies.
Key words: female genital tract malformations / uterine malformations / Müllerian anomalies / embryology / genito-urinary
malformations
Introduction
Objectives
The objectives of this work included the following: (i) to present the
results of a systematic review of the diverse classifications of female
genital tract malformations, unifying concepts and critically analysing
the classifications; (ii) to comment on cases and series where a
correct initial typification would have led to a better treatment, avoiding unnecessary surgery or re-surgeries; and (iii) to propose an
updated classification of genitourinary malformations that integrates
the current embryological and pathogenic concepts and that is
clinically applicable and useful.
Embryological considerations
These considerations are available as Supplementary data SI, but
briefly we must state here that: (i) The appropriate development,
fusion and resorption of the separating wall between both Müllerian
ducts seem to be induced by the Wolffian ducts, which are located
on both sides of the Müllerian ducts and act as guide elements. (ii)
The fused Müllerian ducts form the uterus up to the external cervical
os, and the inducing mesonephric ducts regress cranially, though they
enlarge caudally from the level of the cervical os, form the sinuvaginal
bulbs, incorporate the Müllerian tubercle’s cells and give rise to the
vaginal plate (the cavitation of which is covered by Müllerian cells
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The complex topic of female genital tract malformations should
include malformations that affect the development and morphology
of the Fallopian tubes, uterus, vagina and vulva, with or without associated ovarian, urinary, skeletal or other organ malformations; the topic
should exclude the abnormalities of sexual determination [involving
chromosomal alteration, male histocompatibility (HY) antigen, sexdetermining region of the Y chromosome (SRY) and testis-determining
factor (TDF) gene or the gonads] and sexual differentiation (by abnormal steroidogenesis or pseudohermaphroditisms). Most of the female
genital tract malformations affect the uterus, and therefore, they are
often referred to as uterine or Müllerian (paramesonephric) malformations. However, many of the anomalies that affect the Müllerian
ducts could originate as a mesonephric (Wolffian) anomaly or in the
female gubernaculum.
Female genital tract malformations are frequent but are not always
detected. Therefore, their true prevalence in the general population is
unknown because many are asymptomatic and not noticed (Acién
et al., 2004a), which may create methodological bias (Grimbizis
et al., 2001; Troiano and McCarthy, 2004; Saravelos et al., 2008).
However, the current availability of a wide range of non-invasive diagnostic procedures provides the opportunity to detect anatomical variations more frequently (Troiano and McCarthy, 2004), but a high
index of suspicion in the doctor’s approach is still necessary. In a
recent systematic review that included studies that used more
current diagnostic methods, the mean prevalence of female congenital
malformations in the general population was up to ≏7% (Saravelos
et al., 2008).
Complex malformations of the female genital tract (i.e. not only
uterine or Müllerian malformations) are not very common but they
do occur and are often incorrectly identified, inappropriately treated
and (sometimes) incorrectly reported. The main reasons for frequent
diagnostic delay and/or inappropriate surgery are as follows: (i) not
considering the malformation as a cause of the patient’s clinical symptoms, and (ii) not considering the embryological origin of the different
constituent elements of the genito-urinary tract (Acién, 1992; Acién
et al., 2004a). Embryological hypotheses vary (Koff, 1933; Gruenwald,
1941; Muller et al., 1967a; Forsberg, 1973; Ulfelder and Robboy, 1976;
Marshall and Beisel, 1978; Bok and Drews, 1983; Ludwig, 1998;
Sánchez-Ferrer et al., 2006), and the direct cause of the majority of
anomalies is not known. However, the pathogenesis of the majority
of these anomalies can be correctly explained and understood
through the embryological hypothesis for the female genital tract presented in Acién (1992) and revised in Acién et al. (2004a), SánchezFerrer et al. (2006) and Acién and Acién (2007).
As mentioned already, many women with genital tract malformations are asymptomatic, but others suffer from a wide range of
symptoms and problems that may present at any age and at any
time. In general, symptoms depend on the type of anomaly and the
reproductive age; the following are the most frequently observed: (i)
amenorrhoea in Müllerian ageneses, (ii) intra and postmenstrual dysmenorrhoea in obstructive anomalies, (iii) postmenstrual bleeding in
communicating uteri, (iv) obstetric complications and repeated reproductive losses in the uterine malformations that are due to a lack of
fusion or to a retention of the apposed walls of the paired ducts
(lack of resorption), (v) pelvic tumours that are caused by the
retention of menstrual debris and endometriosis and (vi) extragynaecological problems (urinary, skeletal or auditory).
Naturally, therapeutic approaches are equally variable due to the
diversity of anomalies, clinical presentations and combinations. Moreover, when needed, therapy will always be surgical and will frequently
be simple procedures but therapy is not necessary in the majority of
malformations. However, it is absolutely necessary that the correct
diagnosis is made and, therefore, an adequate classification of the malformation to avoid unnecessary or inadequate interventions is
required. Certainly, the most controversial aspect of genital malformations is that their correct diagnostic evaluation and classification
should be based on aetiopathogenic knowledge of the anomaly and
the suspicion of other associated anomalies that may exist, so
suggesting the appropriate therapeutic strategy. However, most
current classifications do not seem to meet these criteria.
695
Updated classification of genital malformations
Methods
Literature search strategy
A diligent and comprehensive search of PubMed, Scopus, Scirus and ISI
webknowledge was performed for all studies published prior to 15
August 2010, using the following search terms: either ‘classifications’,
‘classes’, ‘embryological’ or ‘embryology’ in combination with either
‘female genital malformations’, ‘female genital tract malformations’,
‘female genital tract anomalies’ ‘müllerian malformations’ or ‘müllerian
anomalies’. The MeSH terms ‘genital malformations’, ‘renal agenesis’,
‘hydrocolpos’, ‘blind or imperforated hemivagina’ and ‘uterine duplication’
were also used in the search of a list of all publications containing these
terms from 1950.
Next, we reviewed the following historical collections: (i) all gynaecology or obstetrics and gynaecology books that were accessible through
our library and included a chapter on female genital tract malformations;
and (ii) all publications about female genital tract malformations prior to
1950 that were accessible through our library. Chapters and papers
were reviewed, as well as their bibliographic references that included
the same terms listed already.
Selection criteria
Initially, only the studies that did not investigate malformations and/or the
embryology of the female genital tract were excluded. Therefore, all of the
studies, case reports and reviews that referred to anomalies or the embryology of the female genital tract and met the previous search term criteria
were considered, which yielded a total of 1260 articles from the listed
electronic databases and historical collections. The titles and abstracts
were analysed to determine whether classification, embryology or embryological origin of genital malformations were mentioned; we also included
general reviews about female genital tract malformations, case reports and
case series where classification, diagnosis or management could be
queried. In addition, references of all relevant articles were hand-searched
for additional citations. The only exclusion criterion for ‘classifications’ was
that there was not a classification of uterine or female genital tract malformations. There were no language restrictions.
Data extraction
Subsequently, we looked for and analysed the 124 full-text articles or book
chapters that included any ‘classification’ of uterine malformations or of
any part of the female genital tract; 20 were published before 1950. All
of these articles were obtained as follows: from their journals or books,
in PDF form from electronic databases, as copies forwarded by their
authors and through interlibrary loan services at our University. Only
five old papers (Cruveilhier, 1842; von Rokitansky, 1859; Kaufmann,
1922; Graves, 1928; De Lee, 1938) could not be obtained, but their classification system was extracted from subsequent comprehensive works
(Ombredanne and Martin, 1905; Piquand, 1910; Taylor, 1943; Jarcho,
1946; Danforth, 1986).
Another 1136 articles or book chapters were excluded from the systematic review for ‘classifications’ for not including any classification of
female genital malformations. Nevertheless, 142 of them were considered
and analysed for genitourinary embryological aspects, and 194 additional
papers were considered for a critical analysis of case reports or case
series where classification, diagnosis or therapeutic management could
be queried. As mentioned already, all relevant articles were analysed for
additional citations.
Critical appraisal
The 124 publications that included ‘classifications’ of female genital tract
malformations were studied and divided into five groups: (i) those that
included a general classification (more or less original) of the malformations of the entire or part (e.g. uterus, vagina or cloacae) of the
female genital tract (49 publications, 15 of which were published before
1950 and 34 after 1950); (ii) those that included a modification of previous
classifications (15 publications, five of which were published before 1950);
(iii) those that exposed cases with more or less complex malformations
without following a determined classification (11 publications); (iv) those
that conducted a critical analysis of the existing classifications without presenting a new one (4 publications); and (v) those that exposed a classification that had been already published by the same or different authors
(45 publications). A Flow Diagram is included (Supplementary data, Fig.
S1a).
Results
Of the 49 publications containing an original classification system, only
13 were global or general classifications, including all the genital or
genitourinary tract; 24 were classifications of uterine anomalies, 3 of
vaginal anomalies, 4 of utero-vaginal anomalies, 2 of segmentary atresias and obstructive anomalies, 2 of concomitant genitourinary malformations and 1 of cloacal anomalies.
Evolutionary analysis of the existing
classifications
The first classifications of female genital tract malformations (and particularly of uterine malformations) from which we collected data were
published in the mid-nineteenth century (Cruveilhier, 1842; Foerster,
1853; von Rokitansky, 1859); these classifications were already based
on embryology and Müllerian duct development. Later, during the first
half of the twentieth century, many other classifications were proposed: Ombredanne and Martin (1905), Strassmann (1907), Piquand
(1910), Forgue and Massabuau (1917), Kaufmann (1922), Stoeckel
and Reifferscheid (1926), De Lee (1938), Way (1945), Jarcho
(1946). However, until 1950, all of the utero-vaginal malformation
classifications were based on the same embryological development
considerations: fusion and development anomalies of the Müllerian
ducts, including rudimentary development, gynatresias and asymmetries (Masson and Kaump, 1937). After 1950, classifications were
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with a cuboidal or paramesonephric epithelium). Then by metaplasic
induction or by epidermization from the sinus, the vagina is covered
by a flat, squamous, stratified epithelium. (iii) Because the ureteral
bud sprouts from the opening in the urogenital sinus of the Wolffian
duct, the absence or distal injury of one of these ducts will give rise
to renal agenesis, a blind or athretic ipsilateral hemivagina and a
uterine anomaly (fusion or resorption defect) due to a failure of the
inducing function of the injured mesonephric or Wolffian duct. (iv)
In the absence of formation and caudal growth of the urogenital
wedge, there is persistent urogenital sinus and then the opening of
the vagina into the sinus can be seen as a vesicovaginal fistula just
underneath and between both uretheral orifices. (v) The female
gubernaculum is likely formed by muscle fibres that are not of a mesonephric or paramesonephric origin and their attachment to the Müllerian ducts allows or induces the fusion and adequate development of
the uterus. Thus, dysfunction of the female gubernacula probably
results in female genital tract malformations.
696
Unifying terms and concepts
Buttram (1983) reported that the terminology used in the past was
imprecise or inconsistently used. Data regarding abortion rates and
obstetric complications in patients with unicornuate or didelphic
uteri were frequently combined under the heading ‘hemiuterus’ and
were not accessible for the evaluation of the individual anomalies. A
similar difficulty arises due to the use of the term ‘double uterus’ to
refer to both bicornuate and septate uteri. In Buttram’s opinion,
these terms should be abandoned. Jones (1953) also mentioned
that there were two disadvantages to all previous classifications
(Taylor, 1943; Way, 1945; Jarcho, 1946). First, most of the classifications employed Latin terminology, which has proved rather impractical because the average obstetrician uses English terms with only
casual use of Latin terms. In addition, ‘bicornuate’, ‘double’ and
‘uterus didelphys’ are applied so interchangeably that it may be
impossible to exactly determine what those terms mean in individual
contexts. Frequently, only the most obvious anomaly is described, and
there is a general tendency to up-grade the anomaly that is observed.
Second, the classifications conveyed little sense of function because
they described the anatomy and were concealed in an unfamiliar
language. Jones (1953) also mentioned that the key to understanding
congenital anomalies lies in their embryology and showed that the two
Müllerian systems canalize and unite into one tract at different times
and at different levels. This allows for numerous combinations,
ranging from the normal single uterus-single cervix-single vagina to
complete ‘uterus didelphys’ with doubling at all levels. Therefore,
after analysing the ‘single’, ‘septate’, ‘bicornuate’ and ‘double’
concepts, Jones compiled the 25 possible combinations of the
uterus– cervix–vagina triad, as shown in Fig. 1A. Jones (1953)
already reported the independence of the duplication anomalies of
the uterus–cervix– vagina, which is similar to what we recently presented (Acién et al., 2009) to clarify the anomalies that were being
published as ‘Müllerian anomalies without a classification’. Those
anomalies correspond to the different transitional forms of Müllerian
anomalies, which range from the didelphys-unicollis uterus to the
normal but bicervical uterus with or without septate vagina
(Fig. 1B), based on the bidirectional theory of septum resorption published by Muller et al. (1967a, b). The embryologic hypothesis of
Müller et al. (1967a) states that fusion and septum resorption begin
at the isthmus and proceed simultaneously in both the cranial and
the caudal directions.
Traditionally, uterus didelphys occurs when there is duplication of
the vagina, cervix and uterus, and Jarcho (1946) previously reported
the confusion with this term because some writers used it only for
cases in which there were two separate parturient canals and bipartite vulva, which is an extremely rare condition. Jarcho (1946) cited
the very unusual case reported by Gemmell and Paterson in 1913.
Therefore, many authors used the term ‘uterus pseudodidelphys’ to
denote duplication of the uterus, cervix and vagina that was not
accompanied by duplication of the vulva. However, this classification
does not seem correct, with the exception of one published case.
In addition, Jarcho (1946) described the ‘uterus duplex bicornis
bicollis’ vagina simplex, which chiefly differs from uterus didelphys
by the absence of duplication of the vagina. Jones (1953) used
the term ‘bicornuate’ uterus to refer to a forked fundus with a
single or septate cervix; he reserved the term ‘double uterus’ for
paired uteri, each of which has a distinct cervix of its own. He
equally distinguishes between ‘double or septate’ cervix, which in
general is easily observed, whereas distinguishing between ‘septate
or double’ vagina is not simple. We think the duplication of the
utero-cervix –vagina, with clear separation of the first two elements,
corresponds to an actual didelphys uterus. However, regarding the
possible discrepancy between the inferior and superior uterine segments with respect to the process of Müllerian fusion (Acién et al.,
2009), the term ‘didelphys’ might also be used in the case of complete separation of the superior segment with a normal inferior one
(didelphys-unicollis uterus). A different anomaly is the bicornuatebicollis uterus, where most of the uterine body and the inferior
uterine segment are fused (see figure from Netter’s atlas in Supplementary data SIII). However, in our opinion, the term ‘double
uterus’ should not be used because it is confusing. The terms
‘two hemiuteri’, a ‘double uterine cavity’ and even ‘utero-vaginal
duplicity’ can be used to refer to the abnormal and complete unification defects of the Müllerian ducts but not as the identification
term or classification of a genital anomaly.
Another controversial point is the differentiation between a bicornuate and septate uterus. While the reproductive results are different,
the traditional diagnostic tool for uterine malformations has been hysterosalpingography (HSG), and this does not enable the distinction
between a bicornuate and subseptate uterus in many cases (which
instead requires laparoscopic observation). For this reason, these
cases have been frequently aggregated under the term ‘double
uteri’, which refers to both the bicornuate uterus and the septate
uterus (Buttram, 1983). In addition, the distinction is as important
for the treatment of symptomatic patients (Strassmann metroplasty
versus hysteroscopic resection). Two-dimensional transvaginal ultrasound, as well as sonohysterography (which is a three-dimensional
computerized ultrasound reconstruction of the uterine cavity) and
magnetic resonance imaging, has ushered in a new era of non-invasive
diagnosis of uterine anomalies. All of these improvements, together
with advances in the field of endoscopy (hysteroscopy and laparoscopy), have greatly increased the efficacy for an accurate, clear and
detailed estimation of the anatomy of the female genital system (Grimbizis and Campo, 2010), though we think the HSG to be the basic tool
in the diagnosis of genital malformations.
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based on different aspects: (i) formation, fusion, resorption and development of the Müllerian ducts, as in the examples shown in Netter’s
atlas (1979). Other classifications distinguish between vertical and
lateral fusion defects of the Müllerian ducts, and others also include
anomalies of the rest of the female genital tract. (ii) on the embryologic origin of the different elements of the genitourinary tract; or (iii)
describe concomitant malformations of the reproductive and urinary
system; or (iv) are functional classifications essentially referring to
the reproductive results; or (v) based on the various altered anatomic
structures or, more appropriately on the anatomic or morphologic
level of the anomaly; or (vi) are partial classifications referring only
to certain anatomical areas, such as vaginal anomalies, those of the
cloacae, etc.
An expanded evolutionary analysis of the existing classifications is
available in Supplementary data SII; and a table showing year,
authors and comments on the articles included in the first four
points of the critical appraisal section is also included.
Acién and Acién
Updated classification of genital malformations
697
Anyway, a collective understanding from the anatomical point of
view that is independent of the methods used for the adequate identification of uterine anomalies is required; uterine anomalies should be
catalogued according to the American Fertility Society (AFS) classification with a few variations, as described in Supplementary data SIII.
Critical analysis of current classifications
As seen already, all of the classifications of uterine malformations from
the mid-nineteenth century to the present have been based on the
Müllerian development processes. Although the variations in the
lateral and vertical fusion defects from Rock and Jones (1977, and
other articles from these authors) and the communicating uteri
(Musset et al., 1968; Toaff et al., 1984) seem to complicate the classifications, they do not significantly change them. Therefore, the
problem is that these exclusively Müllerian embryologic classifications
are not sufficient to explain (and consequently to detect or treat)
other female genitourinary malformations.
The most basic and widely used classification from Jones (1981)
divided the Müllerian anomalies into three groups: agenesis, vertical
fusion defects (obstructive and non-obstructive), and lateral fusion
defects (obstructive and non-obstructive or symmetrical and asymmetrical anomalies). However, Buttram and Gibbons (1979), Buttram
(1983) and Buttram and Reiter (1987) also introduced a classification
system of the Müllerian (uterine) anomalies, which (with few modifications) was then adopted and recommended for general use by
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Figure 1 (A) Variations of uterus – cervix – vagina combinations in the female genital tract malformations according to Jones (1953). (B) Transitional
forms of Müllerian anomalies from the didelphys-unicollis uterus to the normal but bicervical uterus, with or without septate vagina, according to Acién
et al. (2009) (with permission).
698
1985); or (iii) some others consider only punctual aspects, such as
communicating uteri (Musset et al., 1968; Toaff et al., 1984).
More recently, Oppelt et al. (2005) proposed the morphological
and anatomical VCUAM (Vagina, Cervix, Uterus, Adnex, and Associated Malformation) classification. In our opinion, this classification
system is complex and does not suggest the origin or the pathogenic
mechanism of the malformations. Further, the malformation that is
represented by ‘V0,C0,U4a,A#,M#’ is not easily elucidated without
the use of the appropriate tables. Grimbizis and Campo (2010)
reported that this approach provides the opportunity for a precise,
detailed and extremely representative manner of classification.
Indeed, each type of anomaly can be described using this system,
and the clinician will have an accurate idea of each individual’s
genital tract anomaly. However, as Grimbizis and Campo (2010)
mention, ‘the main disadvantage of this system is that it is not
simple or user-friendly’. Additionally, because it is only a nomenclature
for the detected anomalies, it does not make the clinician consider
other undetected but associated anomalies. For example, a unicornuate uterus identified as a ‘V0C0U4aA#M#’ malformation could have a
non-communicating cavitated horn, cervicovaginal atresia, segmentary
atresia or renal agenesis on the contralateral side or even agenesis of
all of the urogenital ridge derivates.
Netter’s atlas includes the classification of uterine anomalies and
also special cases with septate vagina and normal uterus, cases with
atretic hemiuterus and cases with blind hemivagina, which—we currently know—are always associated with ipsilateral renal agenesis. In
addition, in the Stoekel classification, complex malformations related
to hypoplasia or rudimentary cavitated horns in Mayer–Rokitansky –
Kuster–Hauser (MRKH) syndrome cases are illustrated together
with classical anomalies. Alternatively, Musset et al. (1967a) described
four groups or families of malformations of the female genital tract,
and Rossier et al. (2008) compared in a table the ‘classification of
Musset (1964)’ (that we have not found) and that of the AFS
(1988) (mentioned above). Except for the added subtypes, there
are no other differences or advantages between the systems.
Musset et al. (1967b) also identified three possibilities that are
associated with unilateral renal agenesis (i.e. bicornuate bicervical or
didelphys uterus with blind hemivagina, didelphys uterus with
cervico-vaginal atresia, and bicornuate bicervical communicating uteri
with blind hemivagina), thus suggesting a common embryological
origin for these genitourinary malformations. However, in 1953,
Woolf and Allen determined several possible associations of uterine
or genital tract malformation with renal agenesis, which are always
ipsilateral and are considered a consequence of mesonephric
anomaly. If both kidneys are present, this anomaly does not exist.
We believe that Woolf and Allen collected all possibilities of the
association between genital malformations and renal agenesis in an
exceptional body of work from 57 years ago (see Supplementary
data SIV).
In addition, Magee et al. (1979) suggested an embryological classification for genitourinary malformations and identified three types of
unilateral renal agenesis: (i) type I unilateral renal agenesis correlated
with agenesis of all derivates from a urogenital ridge; (ii) type II,
which corresponds to blind hemivagina; and (iii) type III, which
(when correctly identified) corresponds to secondary renal atrophy.
In fact, we currently know that the mesonephric or Wolffian ducts
are as important as the Müllerian ducts and the Müller tubercle. These
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the AFS (currently American Society for Reproductive Medicine,
ASRM) (1988). Buttram (1983) mentioned that classifications used
in the past were helpful but were either too simple or too complex.
Therefore, Buttram and Gibbons (1979) published their classification
that (in their opinion) introduced greater clarity and the potential
for uniformity because it divided the anomalies into groups with
similar clinical manifestations, treatment, and prognosis of pregnancy
outcome. They also reported that their classification was similar to
that suggested by Jarcho in 1946 and later modified by Fenton and
Singh (1952).
The AFS’s (1988) classification consists of seven basic groups that
were essentially analysed based on Müllerian development and its
relationship to fertility: (i) agenesis and hypoplasias, (ii) unicornuate
uteri, (iii) didelphys uteri, (iv) bicornuate uteri, (v) septate uteri, (vi)
arcuate uteri and (vii) anomalies related to diethylstilbestrol-exposure
(DES) syndrome. Additional findings referring to the vagina, cervix, Fallopian tubes, ovaries and urinary system must be separately addressed.
Grimbizis and Campo (2010) reported that the basis for the AFS
classification system is the anatomy of the female genital tract,
especially the uterine anatomy, and that this system is simple, userfriendly and adequately clear. It has been successfully adopted as
the main classification system for almost two decades because the
vast majority of the female congenital malformations are uterine,
which is the basic characteristic for patient grouping that explains
the wide acceptability of the classification scheme. In addition, the
classification of congenital malformations according to the degree of
uterine deformity seems to correlate well with patient prognoses,
mainly in terms of the impact on pregnancy outcome, which is
another notable parameter for explaining the system’s acceptance.
However, many problems are associated with the use of the AFS
classification system, including: (i) it does not encompass many congenital utero-vaginal anomalies and complex genitourinary anomalies,
and it is ineffective for classifying complex anomalies; (ii) class I
includes cases with hypoplasia and/or dysgenesis of the vagina,
cervix, uterus and/or adnexae, and the grouping of these patients
into one category is very general and not functional; and (iii) obstructive anomalies, which are the result of cervical and/or vaginal aplasias
and/or dysplasias, in the presence of either a normal or deformed but
functional uterus are not represented. Therefore, it appears that this
classification system ‘could function as a framework for the description
of anomalies rather than an exhaustive list of all possible anomaly
types’ (Grimbizis and Campo, 2010). In practice, we believe that
the AFS classification, which is well known and similar to the
simpler classification by Jarcho (1946), is adequate for identifying
uterine malformations. Indeed, we retain the AFS classification for
identifying Müllerian anomalies inside the general classification of
genital malformations that we are proposing in this article.
The Jones and Rock classification (Jones, 1981; Rock et al., 1983;
Rock and Schlaff, 1985; Rock, 1992; Jones, 1998) includes complex
malformations among the vertical fusion defects but considers them
as Müllerian anomalies, does not involve their origin or pathogenesis
and does not cover the expectations that a generally accepted classification should. Other classifications have been based on the following:
(i) functional aspects, such as the potential capacity of the uterine
cavity and its musculature (Semmens, 1962) or the reproductive
results (Jones and Jones, 1953); (ii) some contain only obstructive
malformations of the uterus and vagina (Pinsonneault and Goldstein,
Acién and Acién
Updated classification of genital malformations
699
complications. That is the reason why we insist on the embryology and
pathogenesis of genital malformations and on proposing a classification
system which, based on this knowledge, suggests the appropriate
therapeutical strategy.
We agree with Grimbizis and Campo (2010) when referring to the
need for a new classification system that is as clear, easy and exact as
possible; they report that clarity will enable clinicians to develop diagnostic strategies and that anatomy of the female genital tract should be
defined as objectively as possible. However, only exactly describing
the findings, as in the Oppelt et al. (2005) classification, is not adequate. The classification system should suggest other associated
anomalies, and though the ‘clear definitions of each type may help
to avoid subjectivity in the criteria used to recognize and classify
each anomaly’ (Grimbizis and Campo, 2010), it will not avoid the
‘transitional cases’, which normally occur. Often between one
anomaly and another, there are transitional cases that the clinician
will have to decide whether to allocate to one group or another
based on the analysis of the complementary explorations and the
appropriate knowledge of the embryology and pathogenesis. Classifying other transitional cases also depends on the acceptance of the
bidirectional septum resorption theory (Muller et al., 1967a, b)
described already. Therefore, even though Grimbizis and Campo
(2010) state that many publications have reported a number of new
variations of undescribed anomalies with unclear classification (e.g.
septate bicervical uterus), those variations were already addressed
by Jones (1953). Moreover, there are the complex genito-urinary malformations and the malformative combinations mentioned already.
On the basis of the Müllerian development processes, we could
naturally consider as a good classification of the female genital malformations the following:
Comments and proposition
for an updated classification
However, this classification scheme, similar to Jarcho’s classification
(1946) and to that of the AFS (1988), only refers to Müllerian
anomalies and does not address the rest of the elements or the
origin of the female genitourinary tract.
Therefore, after considering the embryological concepts described
in the introduction and in Supplementary data SI and the correlation
between the adult derivatives and female abnormalities of embryonic
urogenital structures; analysing the results of the systematic review;
reviewing the unification of terms and concepts on genital malformations (see also Supplementary data SII); and after critically considering the current classifications, we have the basis: (i) to comment on
series and case reports that can now be better understood as much
in diagnosis as in the adopted therapeutical management, sometimes
with inadequate operation (see Supplementary data SV); and (ii) to
propose a classification system of the female genital tract malformations that necessarily will be a modification and update of our
Frequently, we see patients with genital malformation in which the
therapeutical procedure followed shows that the pre-surgery diagnosis, and usually the post-surgery one, has not been the most
correct. The same is found with many cases reported in the literature.
In Supplementary data SV, we present some examples. This commonly happens because the physician did not bear in mind the embryology of the female genitourinary tract when establishing an
aetiopathogenic diagnosis and, consequently, designing a therapeutical
strategy. Naturally, we do not mean the usual uterine malformations,
but those complex associated genito-urinary anomalies, certain
anomalies of the urogenital sinus and malformative combinations. In
the case of an anomaly that could be easily solved, the treatment
applied might be occasionally so complex that it leads to unnecessary
(i) Anomalies due to total or partial agenesis of one (unicornuate
uterus) or both Müllerian ducts (MRKH syndrome);
(ii) Anomalies due to total or partial absence of fusion, i.e. didelphys
uterus and bicornuate (bicollis and unicollis) uterus;
(iii) Anomalies due to total or partial absence of the reabsorption of
the septum between both Müllerian ducts (septate and subseptate uterus);
(iv) Anomalies due to a lack of later development (hypoplastic
uterus, T-shaped and DES syndrome); and
(v) Segmentary defects and combinations of different anomalies.
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are fundamental for the adequate development of the female genital
tract, especially considering the above mentioned following three
key points: (i) the embryological development of the human vagina
does not proceed from the urogenital sinus and Müllerian ducts
(as classically thought) but from the Wolffian ducts and Müllerian
tubercle; (ii) the appropriate development, fusion and resorption of
the separating wall between both Müllerian ducts is induced by the
Wolffian ducts that are located on both sides and act as guide
elements; and (iii) the Wolffian ducts, in their opening in the urogenital
sinus, sprout and exit through the ureteral bud to form the kidneys in
the metanephros. Therefore, a mesonephric lesion will promote a
vaginal anomaly, a uterine anomaly and ipsilateral renal agenesis
(Acién and Acién, 2010a).
The embryological and clinical classification that we proposed in
1992 (Acién, 1992) and modified in 2004 (Acién et al., 2004a) is
based on these concepts. As shown in Acién et al. (2004a), there
were four locations for the origin of five groups of malformations:
(i) agenesis of an entire urogenital ridge, (ii) mesonephric anomalies,
(iii) Müllerian anomalies, (iv) cloacal anomalies and (v) malformative
combinations. This clinical-embryologic classification may seem
complex but any genital malformation classification that allows their
correct cataloguing and the proper planning of therapeutic strategies
should include the embryological aspects described earlier. This classification system could lead to a better understanding of the pathogenesis of female genital tract anomalies, and we believe that it includes
essentially all genitourinary anomalies and helps as a diagnostic tool,
as well as facilitates the thorough preparation of the surgical correction
of these anomalies. However, our classification system has also been
criticized. Grimbizis and Campo (2010) reported that there is a radical
change in the fundamentals of our classification system from anatomy
(which is the basis for the widely accepted AFS classification system)
to embryogenesis, and this reduces its chances of acceptance. Moreover, our classification system is claimed not to be very simple or
user-friendly. However, the AFS’s classification is only for uterine
anomalies. We think that our system is the most appropriate classification scheme to understand and diagnose a malformation and its
associated anomalies, as well as to achieve more appropriate correction when necessary. The update of our own classification system that
we are proposing in this paper tries to prove it.
700
Acién and Acién
clinical-embryological classification, and that has the fundamental interest of correlation with the clinical presentation, of inducing the search
for other associated anomalies and, in particular, of suggesting the
most simple and appropriate therapeutical procedure.
Proposed updated embryological-clinical
classification for female genitourinary
malformations
(i) Agenesis or hypoplasia of an entire urogenital ridge. In these cases,
there will be an absence of a kidney, functioning ovary, Fallopian
tube, hemiuterus and hemivagina (undetectable) on the same
side. Obviously, we will find a unicornuate uterus on the side
contralateral to the agenesis. Cases may be also associated
with vertebral and/or auditory anomalies (Acién et al., 1991,
2010c) and can present in two different forms:
(a) With bilateral Müllerian agenesis: Rokitansky syndrome with
unilateral renal agenesis; and
(b) Unicornuate uterus with contralateral renal agenesis.
(ii) Mesonephric anomalies with an absence of the Wolffian duct
opening into the urogenital sinus and of the ureteral bud sprouting.
Table I Embryological-clinical classification for female
genito-urinary malformations (revised and updated
from Acién, 1992; and Acién et al., 2004a).
1. Unilateral genito-urinary agenesis or hypoplasia
They are the cases of unicornuate uterus with contralateral renal agenesis
due to agenesis or hypoplasia of an entire urogenital ridge
2. Uterine duplicity (bicornuate or didelphys uterus) with a blind
hemivagina (or unilateral cervico-vaginal atresia) and ipsilateral renal
agenesis
It includes the Herlyn–Werner and Wunderlich syndromes and there can
also be cases of resorption partial of the intervaginal septum
3. Isolated or common uterine or utero-vaginal anomalies
They include the anomalies in the Müllerian development processes also
included in the classification of the American Fertility Society without
other associated anomalies; and also the transverse vaginal septum
4. Accessory uterine masses with an otherwise normal uterus, and other
possible gubernaculum dysfunctions
5. Anomalies of the urogenital sinus
As imperforated hymen, vesico-vaginal fistulas, persistent urogenital sinus,
cloacal anomalies, and other external gastrointestinal or urinary anomalies
6. Malformative combinations
(a) Large hematocolpos in a blind hemivagina, with ipsilateral
renal agenesis;
(b) Gartner’s pseudocyst in the anterolateral wall of the permeable vagina (frequently as a Herlyn-Werner syndrome)
with ipsilateral renal agenesis;
(c) Partial reabsorption of the intervaginal septum (sometimes as
a vaginal septum observed only in the superior half of the
vagina or seen as a buttonhole on the anterolateral wall of
the permeable vagina); and
(d) Complete unilateral vaginal or cervico-vaginal agenesis with
or without communication between both hemiuteri. In the
first case, with communication, the HSG image has the
aspect of a bicornis-unicollis uterus. The second case
(without communication) usually presents with haematometra, retrograde menstruation and endometriosis from a cavitated rudimentary horn, and worsening of symptoms if the
tube or adnexa are removed. Ipsilateral renal agenesis will
also be present.
(iii) Isolated Müllerian anomalies. This group includes the common isolated uterine anomalies that can be classified according to the
AFS (or ASRM) system, with the modifications described
earlier. These Müllerian anomalies can affect the following:
(a) Paramesonephric or Müllerian ducts: tubarian (duplicity, segmentary atresia) and uterine malformations showing agenesis, lack of fusion, absence of reabsorption or lack of later
development, as in the AFS classification:
(1) Agenesis and uterine hypoplasias;
(2) Unicornuate uterus, which is generally accompanied by
athretic rudimentary horn and normal adnex on the
other side, but a rudimentary cavitated and noncommunicated horn can also be present;
(3) Didelphys uterus;
(4) Bicornuate uterus: complete (bicornis-bicollis uterus)
and partial (bicornis-unicollis uterus).
As described already, some cases of bicornis-unicollis
uterus can have a cavitated non-communicating horn,
and its inclusion in a bicornuate or unicornuate uterus
is discussed. Functionally, it is a unicornuate uterus but
it is important to adequately identify the anomaly, to
determine if there is renal agenesis and to decide if surgical correction is required;
(5) Septate uterus: complete and partial or subseptate
uterus;
(6) Arcuate uterus; and
(7) Anomalies related to DES syndrome, also including
hypoplastic and T-shaped uterus.
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In Table I we propose a simplified scheme that includes six groups of
female genitourinary anomalies, and besides a practical and illustrated
application of the proposed classification correlating the ethiopathogenia, the anatomical findings, the diagnosis and the clinical symptoms
are included to aid clinicians in Table II. The therapeutic strategy must
be based on the logical deduction product of the aetiopathogenic
knowledge of the anomaly and on the experience of clinicians
(Acién and Acién, 2004c).
The expanded and updated embryological-clinical classification
includes:
There will be renal agenesis and ipsilateral blind vagina and,
usually, uterine anomaly due to the absence of the ‘inducting’
function of the injured mesonephric duct on the Müllerian duct
(uterine duplicity with/without interseptal or interuterine communication) (Acién and Acién, 2010a). If there is an ectopic
sprout of the ureteral bud, there could then be renal hypoplasia
and ectopic ureter opening into the blind vagina (Acién et al.,
1990, 2004b). These are the most complex malformations,
they may appear as uterine duplicity (didelphys, bicornuate
and, rarely, septate uterus) and present with the following:
701
Updated classification of genital malformations
Table II Illustrated relationship and classification of the pathogenic findings and anatomical figures with symptoms and
pathology name, following an updated embryological and clinical classification of female genital tract malformations.
Aetiopathogenic anomaly
Anatomical findings
Pathology name
Clinical symptoms
1.1 With contralateral müllerian agenesis
Rokitansky syndrome with URA
Primary amenorrhoea
1.2 Without contralateral agenesis
Unicornuate uterus with contralateral
RA
No symptoms.
Reproductive
Breech present
.............................................................................................................................................................................................
1. Unilateral genito-urinary agenesis or hypoplasia
2. Uterine duplicity with a blind hemivagina (or atresia) and ipsilateral RA, showing
Didelphys or bicornuate uterus with
blind hemivagina and ipsilateral RA
Pain. Intra and postmenstrual
dysmenorrhea
Pelvic tumour
Postmenstrual spotting
2.2 Like Gartner’s pseudocyst
Bicornuate communicating uterus,
athretic blind hemivagina and ipsilateral
RA. Herlyn-Werner syndrome
Pain? Cyst in anterolateral wall
of vagina.
Postmenstrual spotting or vaginal
discharge.
2.3 Partial reabsorption of the vaginal septum
Didelphys or bicornis-bicollis uterus
with a short septum or buttonhole, and
URA
No symptoms.
Dyspareunia.
Reproductive.
Breech presentation.
Obstetrical complications
2.4 Complete unilateral vaginal or
cervico-vaginal atresia with communicating
uteri
Bicornis-unicollis uterus with an
anomalous horn and ipsilateral RA
No symptoms
Reproductive
Breech presentation
Obstetrical complications
2.5 Idem, without communicating uteri
Unicornuate uterus with contralateral
unattached but cavitated rudimentary
horn
URA
Pain. Increasing dysmenorrhea
after surgery?
Symptoms as endometriosis
3. Isolated or common uterine or utero-vaginal anomalies, affecting
A. Paramesonephric or müllerian ducts
A.1. Agenesis or hypoplasias
Müllerian agenesis
Primary amenorrhoea
Endometriosis and
criptomenorrhea if cavitated horn
A.2. Unicornuate uterus with atretic
cavitated or non-cavitated rudimentary
horn, or segmentary atresia, or ‘unilateral
Rokitansky syndrome’
Unicornuate uterus; or bicornuate with
cavitated noncommunicated uterine
horn or segmentary atresia
Reproductive.
Breech presentation
Intra or postmenstrual
dysmenorrheal. Endometriosis?
Continued
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2.1 Large hematocolpos, blind hemivagina
702
Acién and Acién
Table II Continued
Aetiopathogenic anomaly
Anatomical findings
Pathology name
Clinical symptoms
A.3. Didelphys uterus
Didelphys uterus
Reproductive
Breech presentation
A.4. Bicornuate uterus.
Bicornis-bicollis uterus and
Reproductive
Bicornis-unicollis uterus
(non-communicating cavitated horn)
Miscarriage. Breech
presentation Inmature delivery.
Retrograde menstruation
A.5. Septate uterus
Septate and subseptate uterus
Reproductive
Miscarriage
Breech presentation Inmature and
premature delivery
A.6. Arcuate uterus
Arcuate uterus
Reproductive losses?
A.7. Anomalies related to DES syndrome
DES syndrome. Hypoplastic and
T-shaped uterus.
Tricavitated uterus
Infertility
.............................................................................................................................................................................................
Eventually, with a non-communicating cavitated
uterine horn
B. Müllerian tubercle
B.1. Complete vaginal or cervico-vaginal
agenesis or atresia
Vaginal or cervico-vaginal atresia
Primary amenorrhoea
Pain
Cryptomenorrhea. Endometriosis
B.2. Segmentary atresias
Complete or incomplete transverse
vaginal septum
Dyspareunia?
Obstetrical problems?
Or primary amenorrhoea and
cryptomennorrhea
Rokitansky or MRKH syndrome
Primary amenorrhoea
Accesory and cavitated uterine masses
with normal uterus.
Didelphic uterus without RA?
Pain
C. Both Müllerian tubercle and ducts
Complete utero-vaginal agenesis
4. Accesory uterine masses and other
gubernaculum dysfunctions
5. Anomalies of the urogenital sinus
Imperforated hymen. Persistent
urogenital sinus. Congenital
vesico-vaginal fistula.
Dysmenorrhea
Tumor
Cryptomenorrhea Pain
Menuria, Hypospadias, Cloacal
fistulas
Continued
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Reproductive losses
703
Updated classification of genital malformations
Table II Continued
Aetiopathogenic anomaly
Anatomical findings
Pathology name
Clinical symptoms
Variable
Variable
.............................................................................................................................................................................................
6. Malformative combinations
URA, unilateral renal agenesis; RA, renal agenesis; MRKH, Mayer–Rokitansky –Kuster–Hauser; DES, diethylstilbestrol.
(b) Müllerian tubercle:
As mentioned earlier, in groups 1 and 2 and in the combinations when
affecting the mesonephric ducts, there will be renal agenesis (Acién
and Acién, 2010a). In all other genital malformation types, there can
also be associated urinary anomalies (e.g. ptosis and horseshoe
kidney) but these may also be found in women without genital
malformations.
sinus determine the adequate formation of the urinary system
and the kidneys. Any lesion of these elements will produce different female genital tract malformations depending on the location
or level of the lesion. The adult derivatives and abnormalities that
correlate with the different urogenital embryonary structures are
also mentioned in Supplementary data SI.
(2) The majority of female genital tract malformations classifications
available from the mid-nineteenth century only considered
uterine (Müllerian) malformations, and they were essentially
based on the lateral fusion defects of the Müllerian ducts. The
AFS classification (1988) proposed by Buttram (1983) and modified from Jarcho (1946) is the most widely used. Other classifications include vertical fusion defects or obstructive anomalies,
communicating uteri, the anatomic typification of the defects
(VCUAM classification) or functional aspects (reproductive
results). Others still are based on genitourinary embryology and
classify the malformations by agenesis of an entire urogenital
ridge, mesonephric anomalies, Müllerian anomalies (without
associated mesonephric anomaly), cloacal anomalies and combinations thereof. The concepts and terms used to describe
female genital tract malformations were analysed and must be
unified.
(3) Addressing the need for an updated classification system of genital
malformations that considers the experience gained from the
application of the current classification systems and that is based
on current embryological and aetiopathogenic knowledge, in this
review an updated embryological-clinical classification of genitourinary malformations that includes correlation with anatomical
findings and clinical presentation (aimed at suggesting the best
therapeutic strategy) was presented.
Supplementary data
Supplementary data are available at http://humupd.oxfordjournals.
org/.
Conclusions
Authors’ roles
(1) Current embryological knowledge shows that the mesonephric or
Wolffian ducts and (likely) the female gubernaculum play a role as
inductors for the adequate development and formation of the
Müllerian ducts. Also, the mesonephric ducts together with the
Müllerian tubercle form the vagina. The ureteral bud sprouting
from the opening of the mesonephric ducts into the urogenital
P.A. designed the study, made the systematic review, tables and
figures, and wrote the manuscript. M.A. participated in the systematic
review, helped with the bibliographic search and reviewed the manuscript. P.A. had full access to all of the data in the study and takes
responsibility for the integrity of the data and the accuracy of the
data analysis.
Downloaded from http://humupd.oxfordjournals.org/ by guest on December 19, 2016
(1) Complete vaginal (or cervico-vaginal) agenesis or atresia;
and
(2) Segmentary atresias as transverse vaginal septum.
(c) Both Müllerian tubercle and ducts: Rokitansky or MRKH
syndrome.
(d) These Müllerian anomalies may display a discrepancy in the
fusion and reabsorption processes between the superior
and inferior uterine segments and segmentary defects.
Some cases are also transitional.
(iv) Gubernaculum dysfunction. These cases are typified by accessory
and cavitated uterine masses and likely by other uterus-like
masses with a completely normal uterus (Acién et al., 2010d).
Other anomalies are probable due to the lack of fusion of the
Müllerian ducts, such as cavitated horns that are unattached in
uterus didelphys, without unilateral renal agenesia.
(v) Anomalies of the urogenital sinus. This category includes cases of
imperforated hymen with persistent urogenital membrane,
congenital vesicovaginal fistulas (pseudo-fistula with menuria),
cloacal anomalies and others that show rectovaginal fistulas and
gastrointestinal or urinary tract anomalies.
(vi) Malformative combinations. These cases involve Wolffian, Müllerian and/or associated cloacae anomalies, sometimes with an
anomaly of the ventral urogenital sinus (Acién et al., 2010b).
Others are cases with mesonephric anomaly on one side and
Müllerian anomaly on the contralateral side, eventually associated
with an anomaly of the urogenital sinus (Acién et al., 2004);
others have presented hereditary renal adysplasia, pulmonar
hypoplasia and Rokitansky syndrome (Acién et al., 2010c).
704
Funding
No funding was either sought or obtained for this study.
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